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http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/8789| Atomically accurate de novo design of single-domain antibodies | |
| Mariana Garcia Sanchez Nathaniel Bennett Joseph Watson Robert Ragotte Andrew Borst DéJenaé See Connor Weidle Riti Biswas Ellen Shrock Philip Leung Buwei Huang Inna Goreshnik Russell Ault Kenneth Carr Benedikt Singer Cameron Criswell Dionne Vafeados Ho Min Kim Susana Vazquez Torres Sidney Chan David Baker | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1101/2024.03.14.585103 | |
| https://www.biorxiv.org/content/10.1101/2024.03.14.585103v1 | |
| Despite the central role that antibodies play in modern medicine, there is currently no way to rationally design novel antibodies to bind a specific epitope on a target. Instead, antibody discovery currently involves time-consuming immunization of an animal or library screening approaches. Here we demonstrate that a fine-tuned RFdiffusion network is capable of designing de novo antibody variable heavy chains (VHH’s) that bind user-specified epitopes. We experimentally confirm binders to four disease-relevant epitopes, and the cryo-EM structure of a designed VHH bound to influenza hemagglutinin is nearly identical to the design model both in the configuration of the CDR loops and the overall binding pose. | |
| bioRxiv | |
| 18-03-2024 | |
| Preimpreso | |
| Inglés | |
| Público en general | |
| VIRUS RESPIRATORIOS | |
| Aparece en las colecciones: | Materiales de Consulta y Comunicados Técnicos |
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| Atomically accurate de novo design of single-domain antibodies.pdf | 13.49 MB | Adobe PDF | Visualizar/Abrir |