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http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/7722| Validation of the RT-LAMP assay in a large cohort of nasopharyngeal swab samples shows that it is a useful screening method for detecting SARS-CoV-2 and its VOC variants | |
| MIREYA CISNEROS VILLANUEVA SUGELA SUSANA BLANCAS ZUGARAZO ALBERTO CEDRO TANDA MAGDALENA RIOS ROMERO Eduardo Hurtado Córdova Oscar Almaraz Rojas DIANA RAQUEL ORTIZ SORIANO VICTOR ALVAREZ HERNANDEZ IVONNE ARRIAGA GUZMÁN LAURA TOLENTINO GARCIA ANTONIA SANCHEZ VIZCARRA LAURA FATIMA LOZADA RODRIGUEZ IRLANDA PERALTA ARRIETA José Enrique Pérez Aquino MARCO ALONSO ANDONEGUI ELGUERA MARIANA CENDEJAS OROZCO ALFREDO MENDOZA VARGAS JUAN PABLO REYES GRAJEDA ABRAHAM CAMPOS ROMERO Jonathan Alcántar Fernández José L. Moreno Camacho JORGE RODRIGUEZ GALLEGOS MARCO ESPARZA-LUNA RUIZ JESUS ORTIZ RAMIREZ MARIANA BENITEZ GONZALEZ LAURA URIBE FIGUEROA Ofelia Angulo ROSAURA RUIZ GUTIERREZ LUIS ALONSO HERRERA MONTALVO ALFREDO HIDALGO MIRANDA | |
| Acceso Abierto | |
| Sin Derechos Reservados | |
| https://doi.org/10.1101/2022.02.15.22270954 | |
| https://www.medrxiv.org/content/10.1101/2022.02.15.22270954v1 | |
| The COVID-19 pandemic is challenging the global supply chain and equipment needed for mass testing with RT-qPCR, the gold standard for SARS-CoV-2 diagnosis. Here, we propose the RT-LAMP assay as an additional strategy for rapid virus diagnosis. However, its validation as a diagnostic method remains uncertain. In this work, we validated the RT-LAMP assay in 1,266 nasopharyngeal swab samples with confirmed diagnosis by CDC 2019-nCoV RT-qPCR. Our cohort was divided, the first (n=984) was used to evaluate two sets of oligonucleotides (S1 and S3) and the second (n=281) to determine whether RT-LAMP could detect samples with several types of variants. This assay can identify positive samples by color change or fluorescence within 40 minutes and shows high concordance with RT-qPCR in samples with CT ≤35. Also, S1 and S3 are able to detect SARS-CoV-2 with a sensitivity of 68.4% and 65.8%, and a specificity of 98.9% and 97.1%, respectively. Furthermore, RT-LAMP assay identified 279 sequenced samples as positive (99.3% sensitivity) corresponding to the Alpha, Beta, Gamma, Delta, Epsilon, Iota, Kappa, Lambda, Mu and Omicron variants. In conclusion, RT-LAMP is able to identify SARS-CoV-2 with good sensitivity and excellent specificity, including all VOC, VOI, VUM and FMV variants. | |
| medRxiv and bioRxiv | |
| 17-02-2022 | |
| Preimpreso | |
| https://www.medrxiv.org/ | |
| Inglés | |
| Epidemia COVID-19 | |
| Público en general | |
| VIRUS RESPIRATORIOS | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos científicos |
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| Validation of the RT LAMP assay in a large cohort of nasopharyngeal swab.pdf | 3.68 MB | Adobe PDF | Visualizar/Abrir |