Por favor, use este identificador para citar o enlazar este ítem:
http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/7662| Comparison of BinaxNOW TM and SARS-CoV-2 qRT-PCR detection of the Omicron Variant from Matched Anterior Nares Swabs | |
| Lena Landaverde Jacquelyn Turcinovic Lynn Doucette Stamm Kevin Gonzales Judy Platt John Connor Catherine Klapperich | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1101/2022.01.31.22270206 | |
| https://www.medrxiv.org/content/10.1101/2022.01.31.22270206v1 | |
| The COVID-19 pandemic has increased the use of rapid antigen tests such as the Abbott BinaxNOWTM COVID-19 Antigen Self-Test. In winter of 2021-2022, the omicron variant surge made it quickly apparent that although rapid diagnostic tests (RDTs) are less sensitive than qRT-PCR, the accessibility, ease of use, and rapid read-outs of RDTs made them a sought after and often sold-out item at local suppliers. Here, we sought to qualify the BinaxNOWTM test for use in our university testing program as a method to rule in positive or rule out negative individuals quickly when they sought care at our priority qRT-PCR testing site. To perform this qualification study, we collected matched additional swabs from individuals attending this test site for standard of care qRT-PCR testing. All matched swabs were tested using the BinaxNOWTM RDT. 48 of 110 samples tested positive for SARS-CoV-2. All samples for which genome sequence could be collected were omicron (41). We observed a calculated sensitivity of 52.1%, specificity of 100%, a positive predictive value (PPV) of 100%, and a negative predictive value (NPV) of 72.9% for the BinaxNOWTM tests (n=110). Sensitivity was improved (75.7%) by changing the qRT-PCR positivity threshold from a CT of 40 to a CT of 30. The ROC curve shows that for qRT-PCR positive CT values between 23-40, the BinaxNOWTM test is of limited value diagnostically. Our results suggest that RDT tests could be used to confirm SARS-CoV-2 infection in individuals with substantial viral load, but that a significant fraction of infected individuals would be missed if we used RDT tests exclusively at this time. | |
| medRxiv and bioRxiv | |
| 01-02-2022 | |
| Preimpreso | |
| https://www.medrxiv.org | |
| Inglés | |
| Epidemia COVID-19 | |
| Público en general | |
| VIRUS RESPIRATORIOS | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos científicos |
Cargar archivos:
| Fichero | Tamaño | Formato | |
|---|---|---|---|
| Comparison of BinaxNOW and SARS Cov2 qRT PCR detection of the Omicron Variant from Matched.pdf | 4.35 MB | Adobe PDF | Visualizar/Abrir |