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http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/7499| Rapid, Affordable and Scalable SARS-CoV-2 Detection from Saliva | |
| Andrew Hayden Marcy Kuentzel Sridar Chittur | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1101/2021.04.01.21254182 | |
| https://www.medrxiv.org/content/10.1101/2021.04.01.21254182v1 | |
| Here we present an inexpensive, rapid, and robust RT-LAMP based SARS-CoV-2 detection method that is easily scalable, enabling point of care facilities and clinical labs to determine results from patients’ saliva directly in 30 minutes for less than $2 a sample. The method utilizes a novel combination of widely available reagents that can be prepared in bulk, plated and frozen and remain stable until samples are received. This innovation dramatically reduces preparation time, enabling high-throughput automation and testing with time to results (including setup) in less than one hour for 96 patient samples simultaneously when using a 384 well format. By utilizing a dual-reporter (phenol red pH indicator for end-point detection and SYTO-9 fluorescent dye for real-time), the assay also provides internal validation of results and redundancy in the event of an instrument malfunction. | |
| medRxiv and bioRxiv | |
| 05-04-2021 | |
| Preimpreso | |
| www.medrxiv.org | |
| Inglés | |
| Epidemia COVID-19 | |
| Público en general | |
| VIRUS RESPIRATORIOS | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos científicos |
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| Fichero | Tamaño | Formato | |
|---|---|---|---|
| Rapid, affordable and Scalable SARS COV 2 Dtection from Saliva.pdf | 2.02 MB | Adobe PDF | Visualizar/Abrir |