Please use this identifier to cite or link to this item: https://covid-19.conacyt.mx/jspui/handle/1000/594
Identification of coronavirus isolated from a patient in Korea with covid-19
Kim, J
Chung, Y
Jo, H
Lee, N
Kim, M
Woo, S
Park, S
Kim, J
Kim, H
Han, M
Acceso Abierto
Atribución-NoComercial-SinDerivadas
Objectives: Following reports of patients with unexplained pneumonia at the end of December 2019 in Wuhan, China, the causative agent was identified as coronavirus (SARS-CoV-2), and the 2019 novel coronavirus disease was named COVID-19 by the World Health Organization. Putative patients with COVID-19 have been identified in South Korea, and attempts have been made to isolate the pathogen from these patients.Methods: Upper and lower respiratory tract secretion samples from putative patients with COVID-19 were inoculated onto cells to isolate the virus. Full genome sequencing and electron microscopy were used to identify the virus.Results: The virus replicated in Vero cells and cytopathic effects were observed. Full genome sequencing showed that the virus genome exhibited sequence homology of more than 99.9% with SARS-CoV-2 which was isolated from patients from other countries, for instance China. Sequence homology of SARS-CoV-2 with SARS-CoV, and MERS-CoV was 77.5% and 50%, respectively. Coronavirus-specific morphology was observed by electron microscopy in virus-infected Vero cells.Conclusion: SARS-CoV-2 was isolated from putative patients with unexplained pneumonia and intermittent coughing and fever. The isolated virus was named BetaCoV/Korea/KCDC03/2020.
Osong Public Health and Research Perspectives
2020
Preimpreso
https://coronavirus.1science.com/item/7b318001ca5b695a455ecdeb83b83d5c5725ef0c
Inglés
VIRUS RESPIRATORIOS
Appears in Collections:Artículos científicos

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