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http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/594| Identification of coronavirus isolated from a patient in Korea with covid-19 | |
| Kim, J Chung, Y Jo, H Lee, N Kim, M Woo, S Park, S Kim, J Kim, H Han, M | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| Objectives: Following reports of patients with unexplained pneumonia at the end of December 2019 in Wuhan, China, the causative agent was identified as coronavirus (SARS-CoV-2), and the 2019 novel coronavirus disease was named COVID-19 by the World Health Organization. Putative patients with COVID-19 have been identified in South Korea, and attempts have been made to isolate the pathogen from these patients.Methods: Upper and lower respiratory tract secretion samples from putative patients with COVID-19 were inoculated onto cells to isolate the virus. Full genome sequencing and electron microscopy were used to identify the virus.Results: The virus replicated in Vero cells and cytopathic effects were observed. Full genome sequencing showed that the virus genome exhibited sequence homology of more than 99.9% with SARS-CoV-2 which was isolated from patients from other countries, for instance China. Sequence homology of SARS-CoV-2 with SARS-CoV, and MERS-CoV was 77.5% and 50%, respectively. Coronavirus-specific morphology was observed by electron microscopy in virus-infected Vero cells.Conclusion: SARS-CoV-2 was isolated from putative patients with unexplained pneumonia and intermittent coughing and fever. The isolated virus was named BetaCoV/Korea/KCDC03/2020. | |
| Osong Public Health and Research Perspectives | |
| 2020 | |
| Preimpreso | |
| https://coronavirus.1science.com/item/7b318001ca5b695a455ecdeb83b83d5c5725ef0c | |
| Inglés | |
| VIRUS RESPIRATORIOS | |
| Aparece en las colecciones: | Artículos científicos |
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