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Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay
James P Broughton.
Xianding Deng.
Guixia Yu.
Clare L Fasching.
Jasmeet Singh.
Jessica Streithorst.
Andrea Granados.
Alicia Sotomayor-Gonzalez.
Kelsey Zorn.
Allan Gopez.
Elaine Hsu.
Wei Gu.
Steven Miller.
Chao-Yang Pan.
Hugo Guevara.
Debra Wadford.
Janice Chen.
Charles Y Chiu.
Acceso Abierto
An outbreak of novel betacoronavirus, SARS-CoV-2 (formerly named 2019-nCoV), began in Wuhan, China in December 2019 and the COVID-19 disease associated with infection has since spread rapidly to multiple countries. Here we report the development of SARS-CoV-2 DETECTR, a rapid (~30 min), low-cost, and accurate CRISPR-Cas12 based lateral flow assay for detection of SARS-CoV-2. We validated this method using contrived reference samples and clinical samples from infected US patients and demonstrated comparable performance to the US CDC SARS-CoV-2 real-time RT-PCR assay.
Appears in Collections:Artículos científicos

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