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http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/4667| SARS-CoV-2 invades host cells via a novel route: CD147-spike protein | |
| Ke Wang. Wei Chen. Yu-Sen Zhou. Jian-Qi Lian. Zheng Zhang. Peng Du. Li Gong. Yang Zhang. Hong-Yong Cui. Jie-Jie Geng. Bin Wang. Xiu-Xuan Sun. Chun-Fu Wang. Xu Yang. Peng Lin. Yong-Qiang Deng. Ding Wei. Xiang-Min Yang. Yu-Meng Zhu. Kui Zhang. Zhao-Hui Zheng. Jin-Lin Miao. Ting Guo. Ying Shi. Jun Zhang. Ling Fu. Qing-Yi Wang. Huijie Bian. Ping Zhu. Zhi-Nan Chen. | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| 10.1101/2020.03.14.988345 | |
| Currently, COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been widely spread around the world; nevertheless, so far there exist no specific antiviral drugs for treatment of the disease, which poses great challenge to control and contain the virus. Here, we reported a research finding that SARS-CoV-2 invaded host cells via a novel route of CD147-spike protein (SP). SP bound to CD147, a receptor on the host cells, thereby mediating the viral invasion. Our further research confirmed this finding. First, in vitro antiviral tests indicated Meplazumab, an anti-CD147 humanized antibody, significantly inhibited the viruses from invading host cells, with an EC50 of 24.86 g/mL and IC50 of 15.16 g/mL. Second, we validated the interaction between CD147 and SP, with an affinity constant of 1.85x10-7M. Co-Immunoprecipitation and ELISA also confirmed the binding of the two proteins. Finally, the localization of CD147 and SP was observed in SARS-CoV-2 infected Vero E6 cells by immuno-electron microscope. Therefore, the discovery of the new route CD147-SP for SARS-CoV-2 invading host cells provides a critical target for development of specific antiviral drugs. | |
| www.biorxiv.org | |
| 2020 | |
| Artículo | |
| https://www.biorxiv.org/content/10.1101/2020.03.14.988345v1.full.pdf | |
| Inglés | |
| VIRUS RESPIRATORIOS | |
| Aparece en las colecciones: | Artículos científicos |
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