Please use this identifier to cite or link to this item: https://covid-19.conacyt.mx/jspui/handle/1000/4208
Rapid Design of a Bait Capture Platform for Culture- and Amplification-Free Next-Generation Sequencing of SARS-CoV-2
Nasir Jalees.
Speicher David.
Kozak Robert.
Poinar Hendrik.
Miller Matthew.
McArthur Andrew.
Acceso Abierto
Atribución-NoComercial-SinDerivadas
10.20944/preprints202002.0385.v1
SARS-CoV-2 is a novel betacoronavirus and the aetiological agent of the current COVID-19 outbreak that originated in Hubei Province, China. While polymerase chain reaction is the front-line tool for SARS-CoV-2 surveillance, application of amplification-free and culture-free methods for isolation of SARS-CoV-2 RNA, partnered with next-generation sequencing, would provide a useful tool for both surveillance and research of SARS-CoV-2. We here release into the public domain a set of bait capture hybridization probe sequences for enrichment of SARS-CoV-2 RNA from complex biological samples. These probe sequences have been designed using rigorous bioinformatics methods to provide sensitivity, accuracy, and minimal off-target hybridization. Probe design was based on existing, validated approaches for detecting antimicrobial resistance genes in complex samples and it is our hope that this SARS-CoV-2 bait capture platform, once validated by those with samples in hand, will be of aid in combating the current outbreak.
www.preprints.org
2020
Artículo
https://www.preprints.org/manuscript/202002.0385/v1/download
Inglés
VIRUS RESPIRATORIOS
Appears in Collections:Artículos científicos

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