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http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/4198| The Nucleocapsid Protein of SARS-CoV-2 Abolished Pluripotency in Human Induced Pluripotent Stem Cells | |
| Zebin Lin. Qian Fang. Jinlian Mai. Lishi Zhou. Yu Qian. Tian Cai. Zhenhua Chen. Ping Wang. Bin Lin. | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| 10.1101/2020.03.26.010694 | |
| The COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is raging across the world, leading to a mortality rate of 3.4%. As a potential vaccine and therapeutic target, the nucleocapsid protein of SARS-CoV-2 (nCoVN) functions in packaging the viral genome and viral self-assembly. To investigate the biological effect of nCoVN to human induced pluripotent stem cells (iPSC), genetically engineered iPSC overexpressing nCoVN (iPSC-nCoVN) were generated by lentiviral expression systems. Unexpectedly, the morphology and proliferation rate of iPSC were changed after nCoVN expressing for two weeks. The pluripotency markers SSEA4 and TRA-1-81 were not detectable in iPSC-nCoVN. Meanwhile, iPSC-nCoVN lost the ability for differentiation into cardiomyocytes when using a routine differentiation protocol. Our data suggested that nCoVN disrupted the pluripotent properties of iPSC and turned them into fibroblasts, which provided a new insight to the pathogenic mechanism of SARS-CoV-2. | |
| www.biorxiv.org | |
| 2020 | |
| Artículo | |
| https://www.biorxiv.org/content/10.1101/2020.03.26.010694v2.full.pdf | |
| Inglés | |
| VIRUS RESPIRATORIOS | |
| Aparece en las colecciones: | Artículos científicos |
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| 1106021.pdf | 1.14 MB | Adobe PDF | Visualizar/Abrir |