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http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/4147| An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage | |
| Anna S Fomsgaard. Maiken W Rosenstierne. | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| 10.1101/2020.03.27.20044495 | |
| Abstract The World Health Organisation has declared a pandemic caused by the newly discovered SARS-CoV-2. Due to growing demand for reagents used for SARS-CoV-2 RNA extraction for subsequent molecular diagnostics, there is a worldwide risk of kit- and/or reagent-shortages for extraction. With a detection sensitivity of 97.4% (95% CI=86.2-99.9%), we describe a simple, fast, alternative workflow for molecular detection of SARS-CoV-2, where samples are simply heat-processed for 5 minutes at 98{degrees}C prior to the RT-qPCR reaction. | |
| www.medrxiv.org | |
| 2020 | |
| Artículo | |
| https://www.medrxiv.org/content/10.1101/2020.03.27.20044495v1.full.pdf | |
| Inglés | |
| VIRUS RESPIRATORIOS | |
| Aparece en las colecciones: | Artículos científicos |
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| 1105898.pdf | 592.82 kB | Adobe PDF | Visualizar/Abrir |