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SARS-CoV-2 detection from nasopharyngeal swab samples without RNA extraction
Carolina Beltran-Pavez.
Chantal L Marquez.
Gabriela Munoz.
Fernando Valiente-Echeverria.
Aldo Gaggero.
Ricardo Soto-Rifo.
Gonzalo P Barriga.
Acceso Abierto
Atribución-NoComercial-SinDerivadas
10.1101/2020.03.28.013508
The ongoing COVID-19 pandemic has reached more than 200 countries and territories worldwide. Given the large requirement of SARS-CoV-2 diagnosis and considering that RNA extraction kits are in short supply, we investigated whether two commercial RT-qPCR kits were compatible with direct SARS-CoV-2 detection from nasopharyngeal swab samples. We show that one of the tested kits is fully compatible with direct SARS-CoV-2 detection suggesting that omission of an RNA extraction step should be considered in SARS-CoV-2 diagnosis.
www.biorxiv.org
2020
Artículo
https://www.biorxiv.org/content/10.1101/2020.03.28.013508v1.full.pdf
Inglés
VIRUS RESPIRATORIOS
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