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http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/4066| Structural analysis of SARS-CoV-2 andprediction of the human interactome | |
| Andrea Vandelli. Michele Monti. Edoardo Milanetti. Riccardo Delli Ponti. Gian Gaetano Tartaglia. | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| 10.1101/2020.03.28.013789 | |
| We calculated the structural properties of >2500 coronaviruses and computed >100000 human protein interactions with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Using the CROSS method, we found that the SARS-CoV-2 region encompassing nucleotides 23000 - 24000 is highly conserved at the structural level, while the region upstream varies significantly. These two sequences are important for viral infection as they code for a domain of the viral protein Spike S interacting with the human receptor angiotensin-converting enzyme 2 (ACE2) and, in the close homologue from Middle East respiratory syndrome coronavirus (MERS-CoV), sialic acids. We predict highly structured regions at the 5 and 3 where our calculations indicate strong propensity to bind to human proteins involved in viral replication. Using the catRAPID method, we identified that the 5 interacts with double-stranded RNA-specific editase 1 ADARB1, 2-5A-dependent ribonuclease RNASEL, ATP-dependent RNA helicase DDX1 and A-kinase anchor protein 8-like AKAP8L, in addition to >10 high-confidence candidate partners. These interactions, also implicated in HIV replication, should be further investigated for a better understanding of host-virus interaction mechanisms. | |
| www.biorxiv.org | |
| 2020 | |
| Artículo | |
| https://www.biorxiv.org/content/10.1101/2020.03.28.013789v5.full.pdf | |
| Inglés | |
| VIRUS RESPIRATORIOS | |
| Aparece en las colecciones: | Artículos científicos |
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| 1105764.pdf | 2.05 MB | Adobe PDF | Visualizar/Abrir |