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http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/404| Molecular epidemiology of human rhinovirus from one-year surveillance within a school setting in rural coastal Kenya | |
| Elijah Gicheru Alex Gichuki D James Nokes Martha M Luka Patrick K Munywoki Nelson Kibinge Charles N Agoti Grieven P Otieno Irene Adema Everlyn Kamau | |
| Novel Coronavirus | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| 10.1101/2020.03.09.20033019 | |
| Background: Human rhinovirus (HRV) is the most common cause of the common cold but may also lead to more severe respiratory illness in vulnerable populations. The epidemiology and genetic diversity of HRV within a school setting have not been described. Objective: To characterise HRV molecular epidemiology among children attending primary school in rural coastal Kenya. Methods: Between May 2017 to April 2018, over three school terms, we collected 1859 nasopharyngeal swab (NPS) samples from pupils and teachers with symptoms of acute respiratory infection in a public primary school in rural coastal Kenya. The samples were tested for HRV using real-time RT-PCR. HRV positive samples were sequenced in the VP4/VP2 coding region for species and genotype classification. Results: A total of 307 NPS (16.4%) from 164 individuals were HRV positive, and 253 (82.4%) were successfully sequenced. The proportion of HRV in the lower primary classes was higher (19.8%) than upper primary classes (12.2%), p-value <0.001. HRV-A was the most common species (134/253, 53.0%), followed by HRV-C (73/253, 28.9%) then HRV-B (46/253, 18.2%). Phylogenetic analysis identified 47 HRV genotypes. The most common genotypes were A2 and B70. Numerous (up to 22 in one school term) genotypes circulated simultaneously and no genotype was detected in all three school terms. Conclusion: HRV was frequently detected among school-going children with mild ARI symptoms, and particularly in the younger age groups (<5-year-olds). Multiple HRV introductions were observed characterized by the considerable genotype diversity. ### Competing Interest Statement The authors have declared no competing interest. ### Funding Statement This work was supported by the Wellcome Trust through a Wellcome Senior Investigator Award to the last author (102975). This work was done in partial fulfilment of the first author's postgraduate diploma studentship. The studentship was supported through the DELTAS Africa Initiative [DEL-15-003]. The DELTAS Africa Initiative is an independent funding scheme of the African Academy of Sciences (AAS)'s Alliance for Accelerating Excellence in Science in Africa (AESA) and supported by the New Partnership for Africa's Development Planning and Coordinating Agency (NEPAD Agency) with funding from the Wellcome Trust [107769/Z/10/Z] and the UK government. The views expressed in this publication are those of the author(s) and not necessarily those of AAS, NEPAD Agency, Wellcome Trust or the UK government ### Author Declarations All relevant ethical guidelines have been followed; any necessary IRB and/or ethics committee approvals have been obtained and details of the IRB/oversight body are included in the manuscript. Yes All necessary patient/participant consent has been obtained and the appropriate institutional forms have been archived. Yes I understand that all clinical trials and any other prospective interventional studies must be registered with an ICMJE-approved registry, such as ClinicalTrials.gov. I confirm that any such study reported in the manuscript has been registered and the trial registration ID is provided (note: if posting a prospective study registered retrospectively, please provide a statement in the trial ID field explaining why the study was not registered in advance). Yes I have followed all appropriate research reporting guidelines and uploaded the relevant EQUATOR Network research reporting checklist(s) and other pertinent material as supplementary files, if applicable. Yes The replication data and analysis scripts for this manuscript shall be made available at the Harvard Dataverse: (https://dataverse.harvard.edu/dataverse/vec). Some of the clinical dataset contains potentially identifying information on participants and is stored under restricted access. Requests for access to the restricted dataset should be made to the Data Governance Committee (dgc@kemri-wellcome.org) of the KEMRI-Wellcome Trust Research Programme. | |
| Cold Spring Harbor Laboratory Press | |
| 2020 | |
| Preimpreso | |
| https://www.medrxiv.org/content/10.1101/2020.03.09.20033019v2 | |
| Inglés | |
| VIRUS RESPIRATORIOS | |
| Aparece en las colecciones: | Artículos científicos |
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