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http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/3474| An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020. | |
| Fomsgaard Anna S. Rosenstierne Maiken Worsøe. | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| 10.2807/1560-7917.es.2020.25.14.2000398 | |
| The World Health Organization has declared COVID-19 caused by the newly discovered SARS-CoV-2 a pandemic. Due to growing demand for reagents and/or kits to extract SARS-CoV-2 RNA for subsequent RT-qPCR diagnostics, there is a worldwide risk of shortages. With a detection sensitivity of 97.4% (95% CI: 86.2-99.9%), we describe a simple, fast, alternative workflow for molecular detection of SARS-CoV-2, where samples are simply heat-processed for 5 min at 98 °C before a commonly-used RT-qPCR procedure. | |
| Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin | |
| 2020 | |
| Artículo | |
| https://www.eurosurveillance.org/deliver/fulltext/eurosurveillance/25/14/2000398_ROSENSTIERNE_Supplementary_Data.pdf?itemId=/content/suppdata/10.2807/1560-7917.ES.2020.25.14.2000398-1&mimeType=pdf | |
| Inglés | |
| VIRUS RESPIRATORIOS | |
| Aparece en las colecciones: | Artículos científicos |
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