Please use this identifier to cite or link to this item: https://covid-19.conacyt.mx/jspui/handle/1000/3419
Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction
Siu-Ying Lau
Pui Wang
Bobo Wing-Yee Mok
HIN CHU
Andrew Chak Yiu Lee
Pin Chen
Kwok Hung Chan
Wenjun Song
Zhiwei Chen
Kelvin To
Jasper Fuk-Woo Chan
Kwok_yung Yuen
Honglin Chen
Acceso Abierto
Atribución
https://doi.org/10.1080/22221751.2020.1756700
COVID-19
SARS-CoV-2
Spike mutant
Spike S1/S2 mutant
The emergence of SARS-CoV-2 has led to the current global coronavirus pandemic and more than one million infections since December 2019. The exact origin of SARS-CoV-2 remains elusive, but the presence of a distinct motif in the S1/S2 junction region suggests the possible acquisition of cleavage site(s) in the spike protein that promoted cross-species transmission. Through plaque purification of Vero-E6 cultured SARS-CoV-2, we found a series of variants which contain 15-30-bp deletions (Del-mut) or point mutations respectively at the S1/S2 junction. Examination of the original clinical specimen from which the isolate was derived, and 26 additional SARS-CoV-2 positive clinical specimens, failed to detect these variants. Infection of hamsters shows that one of the variants (Del-mut-1) which carries deletion of 10 amino acids (30bp) does not cause the body weight loss or more severe pathological changes in the lungs that is associated with wild type virus infection. We suggest that the unique cleavage motif promoting SARS-CoV-2 infection in humans may be under strong selective pressure, given that replication in permissive Vero-E6 cells leads to the loss of this adaptive function. It would be important to screen the prevalence of these variants in asymptomatic infected cases. The potential of the Del-mut variants as an attenuated vaccine or laboratory tool should be evaluated.
Emerging Microbes & Infections
04-05-2020
Artículo
https://www.tandfonline.com/doi/full/10.1080/22221751.2020.1756700
Inglés
Epidemia COVID-19
Investigadores
VIRUS RESPIRATORIOS
Versión publicada
publishedVersion - Versión publicada
Appears in Collections:Artículos científicos

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