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http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/3051| Scaling diagnostics in times of COVID-19: Rapid prototyping of 3D-printed water circulators for Loop-mediated Isothermal Amplification (LAMP) and detection of SARS-CoV-2 virus | |
| Everardo Gonzalez-Gonzalez. Itzel Montserrat Lara-Mayorga. Andres Garcia-Rubio. Carlos Ezio Garciamendez-Mijares. Gilberto Emilio Guerra-Alvarez. German Garcia-Martinez. Juan Andres Aguayo-Hernandez. Yu-Shrike Zhang. Sergio Omar Martinez-Chapa. Grissel Trujillo-de Santiago. Mario Moises Alvarez. | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| 10.1101/2020.04.09.20058651 | |
| By the first week of April 2020, more than 1,500,000 positive cases of COVID-19 and more than 50,000 deaths had been officially reported worldwide. While developed countries such as the USA, Italy, England, France, Spain, and Germany struggle to mitigate the propagation of SARS-CoV-2, the COVID-19 pandemic arrived in Latin America, India, and Africa -territories in which the mounted infrastructure for diagnosis is greatly underdeveloped. An actual epidemic emergency does not provide the required timeframe for testing new diagnostic strategies; therefore, the first line of response must be based on commercially and readily available resources. Here, we demonstrate the combined use of a three-dimensional (3D)-printed incubation chamber for commercial Eppendorf PCR tubes, and a colorimetric embodiment of a loop-mediated isothermal amplification (LAMP) reaction scheme for the detection of SARS-CoV-2 nucleic acids. We used this strategy to detect and amplify SARS-CoV-2 DNA sequences using a set of in-house designed initiators that target regions encoding the N protein. We were able to detect and amplify SARS-CoV-2 nucleic acids in the range of ~625 to 2 X 105 DNA copies by this straightforward method. The accuracy and simplicity of this diagnostics strategy may provide a cost-efficient and reliable alternative for use during the COVID-19 pandemics, particularly in underdeveloped regions were the availability of RT-qPCR instruments may be limited. Moreover, the portability, ease of use, and reproducibility of this strategy make it a reliable alternative for deployment of point-of-care SARS-CoV-2 detection efforts during the pandemics. | |
| www.medrxiv.org | |
| 2020 | |
| Artículo | |
| https://www.medrxiv.org/content/10.1101/2020.04.09.20058651v1.full.pdf | |
| Inglés | |
| VIRUS RESPIRATORIOS | |
| Aparece en las colecciones: | Artículos científicos |
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