Please use this identifier to cite or link to this item: https://covid-19.conacyt.mx/jspui/handle/1000/2970
A method to prevent SARS-CoV-2 IgM false positives in gold immunochromatography and enzyme-linked immunosorbent assays.
Q Wang.
Q Du.
B Guo.
D Mu.
X Lu.
Q Ma.
Y Guo.
L Fang.
B Zhang.
G Zhang.
X Guo.
Acceso Abierto
Atribución-NoComercial-SinDerivadas
10.1128/JCM.00375-20
Objective: To investigate the interference factors that lead to false-positive novel coronavirus (SARS-CoV-2) IgM detected using gold immunochromatography assay (GICA) and enzyme-linked immunosorbent assay (ELISA) and the corresponding solutions.Methods: GICA and ELISA were used to detect SARS-CoV-2 IgM in 86 serum samples, including 5 influenza A virus (Flu A) IgM-positive sera, 5 influenza B virus (Flu B) IgM-positive sera, 5 Mycoplasma pneumoniae IgM-positive sera, 5 Legionella pneumophila IgM-positive sera, 6 sera of HIV infection patients, 36 rheumatoid factor IgM (RF-IgM)-positive sera, 5 sera from hypertensive patients, 5 sera from diabetes mellitus patients, and 14 sera from novel coronavirus infection disease (COVID-19) patients. The interference factors causing false-positive reactivity in the two methods were analyzed, and the urea dissociation test was employed to dissociate the SARS-CoV-2 IgM-positive serum using the best dissociation concentration.Results: Two methods detected positive SARS-CoV-2 IgM in 22 middle-high level RF-IgM-positive sera and 14 sera from COVID-19 patients; the other 50 sera were negative. When urea dissociation concentration was 6 mol/L, SARS-CoV-2 IgM were positive in 1 middle-high level RF-IgM-positive sera and in 14 COVID-19 patient sera detected using GICA. When urea dissociation concentration was 4 mol/L and the avidity index (AI) lower than 0.371 was set to negative, SARS-CoV-2 IgM were positive in 3 middle-high level RF-IgM-positive sera and in 14 COVID-19 patient sera detected using ELISA.Conclusion: Middle-high level of RF-IgM could lead to false-positive reactivity of SARS-CoV-2 IgM detected using GICA and ELISA, and urea dissociation tests would be helpful in reducing false-positive results of SARS-CoV-2 IgM.
Journal of clinical microbiology
2020
Artículo
https://jcm.asm.org/content/jcm/early/2020/04/09/JCM.00375-20.full.pdf
Inglés
VIRUS RESPIRATORIOS
Appears in Collections:Artículos científicos

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