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http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/259| Molecular malaria surveillance using a novel protocol for extraction and analysis of nucleic acids retained on used rapid diagnostic tests | |
| Carlos Cortes Falla Wonder P. Phiri Guillermo A. Garcia Marcel Tanner Claudia Daubenberger Salome Hosch Olivier Tresor Donfack Charlene Aya Yoboue Silvan Kraehenbuehl Anna Deal Glenda Cosi Linda Gondwe Carl Maas Bonifacio Manguire Nlavo Salim Abdulla Grace Mwangoka Heavenlight Masuki Nahya Salim Maxmillian Mpina Jongo Said Stephen L. Hoffman Tobias Schindler Etienne A Guirou | |
| Novel Coronavirus | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| 10.1101/2020.02.17.20023960 | |
| The use of malaria rapid diagnostic tests (RDTs) as a source for nucleic acids that can be analyzed via nucleic acid amplification techniques has several advantages, including minimal amounts of blood, sample collection, simplified storage and shipping conditions at room temperature. We have systematically developed and extensively evaluated a procedure to extract total nucleic acids from used malaria RDTs. The co-extraction of DNA and RNA molecules from small volumes of dried blood retained on the RDTs allows detection and quantification of P. falciparum parasites from asymptomatic patients with parasite densities as low as 1 Pf/μL blood using reverse transcription quantitative PCR. Based on the extraction protocol we have developed the ENAR (Extraction of Nucleic Acids from RDTs) approach; a complete workflow for large-scale molecular malaria surveillance. Using RDTs collected during a malaria indicator survey we demonstrated that ENAR provides a powerful tool to analyze nucleic acids from thousands of RDTs in a standardized and high-throughput manner. We found several, known and new, non synonymous single nucleotide polymorphisms in the propeller region of the kelch 13 gene among isolates circulating on Bioko Island, Equatorial Guinea. ### Competing Interest Statement SL Hoffman is salaried and full-time employee of Sanaria Inc, the developer and sponsor of Sanaria® PfSPZ Vaccine. He was not responsible for the collection, recording or entry of the parasitological data used in this study. The other authors have no conflicts of interest. ### Clinical Trial NCT02613520, [NCT03420053][1] ### Funding Statement This study was funded by a publicprivate partnership, the Equatorial Guinea Malaria Vaccine Initiative (EGMVI), made up of the Government of Equatorial Guinea, Marathon EG Production Limited, Noble Energy, and Atlantic Methanol Production Company. ### Author Declarations All relevant ethical guidelines have been followed; any necessary IRB and/or ethics committee approvals have been obtained and details of the IRB/oversight body are included in the manuscript. Yes All necessary patient/participant consent has been obtained and the appropriate institutional forms have been archived. Yes I understand that all clinical trials and any other prospective interventional studies must be registered with an ICMJE-approved registry, such as ClinicalTrials.gov. I confirm that any such study reported in the manuscript has been registered and the trial registration ID is provided (note: if posting a prospective study registered retrospectively, please provide a statement in the trial ID field explaining why the study was not registered in advance). Yes I have followed all appropriate research reporting guidelines and uploaded the relevant EQUATOR Network research reporting checklist(s) and other pertinent material as supplementary files, if applicable. Yes The datasets supporting the conclusions of this article are included within the article. [1]: /lookup/external-ref?link_type=CLINTRIALGOV&access_num=NCT03420053&atom=%2Fmedrxiv%2Fearly%2F2020%2F02%2F20%2F2020.02.17.20023960.atom | |
| Cold Spring Harbor Laboratory Press | |
| 2020 | |
| Preimpreso | |
| https://www.medrxiv.org/content/10.1101/2020.02.17.20023960v1 | |
| Inglés | |
| VIRUS RESPIRATORIOS | |
| Aparece en las colecciones: | Artículos científicos |
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| molecular malaria.pdf | 705.59 kB | Adobe PDF | Visualizar/Abrir |