Por favor, use este identificador para citar o enlazar este ítem:
http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/2533| Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor | |
| Seo Giwan. Lee Geonhee. Kim Mi Jeong. Baek Seung-Hwa. Choi Minsuk. Ku Keun Bon. Lee Chang-Seop. Jun Sangmi. Park Daeui. Kim Hong Gi. Kim Seong-Jun. Lee Jeong-O. Kim Bum Tae. Park Edmond Changkyun. Kim Seung Il. | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| 10.1021/acsnano.0c02823 | |
| Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious disease caused by acute respiratory syndrome coronavirus 2 (SARS-CoV-2, previously called 2019-nCoV). Based on the rapid increase in the rate of human infection, the World Health Organization (WHO) has classified the COVID-19 outbreak as a pandemic. Because no specific drugs or vaccines for COVID-19 are yet available, early diagnosis and management are crucial for containing the outbreak. Here, we report a field-effect transistor (FET)-based biosensing device for detecting SARS-CoV-2 in clinical samples. The sensor was produced by coating graphene sheets of FET with a specific antibody against SARS-CoV-2 spike protein. The performance of the sensor was determined using antigen protein, cultured virus, and nasopharyngeal swab specimens from COVID-19 patients. Our FET device could detect SARS-CoV-2 spike protein at concentrations of 1 fg/ml in PBS and 100 fg/ml clinical transport medium. In addition, the FET sensor successfully detected SARS-CoV-2 in culture medium (limit of detection [LOD]: 1.6 x 101 pfu/ml) and clinical samples (LOD: 2.42 x 102 copies/ml). Thus, we have successfully fabricated a promising FET biosensor for SARS-CoV-2; our device is a highly sensitive immunological diagnostic method for COVID-19 that requires no sample pretreatment or labeling. | |
| ACS Nano | |
| 2020 | |
| Artículo | |
| https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7172500/pdf/main.pdf | |
| Inglés | |
| VIRUS RESPIRATORIOS | |
| Aparece en las colecciones: | Artículos científicos |
Cargar archivos:
| Fichero | Tamaño | Formato | |
|---|---|---|---|
| 1102042.pdf | 12.46 MB | Adobe PDF | Visualizar/Abrir |