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http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/2400| Structural basis to design multi-epitope vaccines against Novel Coronavirus 19 (COVID19) infection, the ongoing pandemic emergency: an in silico approach | |
| Sukrit Srivastava. Sonia Verma. Mohit Kamthania. Rupinder Kaur. Ruchi Kiran Badyal. Ajay Kumar Saxena. Ho-Joon Shin. Michael Kolbe. Kailash Pandey. | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| 10.1101/2020.04.01.019299 | |
| The 2019 novel coronavirus (COVID19 / Wuhan coronavirus), officially named as Severe Acute Respiratory Syndrome Coronavirus 2 (SARS CoV 2), is a positive sense single stranded RNA coronavirus. SARS CoV 2 causes the contagious COVID19 disease also known as 2019 nCoV acute respiratory disease and has led to the ongoing 2019 20 pandemic COVID19 outbreak. The effective counter measures against SARS CoV 2 infection require the design and development of specific and effective vaccine candidate. In the present study, we have screened and shortlisted 38 CTL, 33 HTL and 12 B cell epitopes from the eleven Protein sequences of SARS CoV 2 by utilizing different in silico tools. The screened epitopes were further validated for their binding with their respective HLA allele binders and TAP (Transporter associated with antigen processing) molecule by molecular docking. The shortlisted screened epitopes were further utilized to design novel two multi epitope vaccines (MEVs) composed of CTL, HTL and B cell epitopes overlaps with potential to elicit humoral as well as cellular immune response against SARS CoV 2. To enhance the immune response for our vaccine design, truncated (residues 10 153) Onchocerca volvulus activation associated secreted protein 1 (Ov ASP 1) has been utilized as an adjuvant at N terminal of both the MEVs. Further molecular models for both the MEVs were prepared and validated for their stable molecular interactions with Toll Like Receptor 3 (TLR 3). The codon optimized cDNA of both the MEVs were further analyzed for their potential of high level of expression in a human cell line. The present study is very significant in terms of molecular designing of prospective CTL and HTL vaccine against SARS CoV 2 infection with the potential to elicit cellular as well as humoral immune response. | |
| www.biorxiv.org | |
| 2020 | |
| Artículo | |
| https://www.biorxiv.org/content/10.1101/2020.04.01.019299v2.full.pdf | |
| Inglés | |
| VIRUS RESPIRATORIOS | |
| Aparece en las colecciones: | Artículos científicos |
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