Por favor, use este identificador para citar o enlazar este ítem: http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/1991
Single Nucleus Multiomic Profiling Reveals Age-Dynamic Regulation of Host Genes Associated with SARS-CoV-2 Infection
Allen Wang.
Joshua A Chiou.
Olivier B Poirion.
Justin Buchanan.
Michael J Valdez.
Jamie M Verheyden.
Xiaomeng Hou.
Jacklyn M Newsome.
Parul Kudtarkar.
Dina A Faddah.
Kai Zhang.
Randee E Young.
Justinn Barr.
Ravi Misra.
Heidie Huyck.
Lisa Rogers.
Cory Poole.
Gloria Pryhuber.
Kyle J Gaulton.
Sebastian Preissl.
Xin Sun.
- NHLBI LungMap Consortium.
Acceso Abierto
Atribución-NoComercial-SinDerivadas
10.1101/2020.04.12.037580
Respiratory failure is the leading cause of COVID-19 death and disproportionately impacts adults more than children. Here, we present a large-scale snATAC-seq dataset (90,980 nuclei) of the human lung, generated in parallel with snRNA-seq (46,500 nuclei), from healthy donors of ~30 weeks, ~3 years and ~30 years of age. Focusing on genes implicated in SARS-CoV-2 cell entry, we observed an increase in the proportion of alveolar epithelial cells expressing ACE2 and TMPRSS2 in adult compared to young lungs. Consistent with expression dynamics, 10 chromatin peaks linked to TMPRSS2 exhibited significantly increased activity with age and harbored IRF and STAT binding sites. Furthermore, we identified 14 common sequence variants in age-increasing peaks with predicted regulatory function, including several associated with respiratory traits and TMPRSS2 expression. Our findings reveal a plausible contributor to why children are more resistant to COVID-19 and provide an epigenomic basis for transferring this resistance to older populations.
www.biorxiv.org
2020
Artículo
https://www.biorxiv.org/content/10.1101/2020.04.12.037580v1.full.pdf
Inglés
VIRUS RESPIRATORIOS
Aparece en las colecciones: Artículos científicos

Cargar archivos:


Fichero Tamaño Formato  
1100778.pdf13.19 MBAdobe PDFVisualizar/Abrir