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http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/1878
Optimizing RT-PCR detection of SARS-CoV-2 for developing countries using pool testing | |
Farfan Mauricio. Torres Juan. ORyan Miguel. Olivares Mauricio. Gallardo Pablo. Salas Carolina. | |
Acceso Abierto | |
Atribución-NoComercial-SinDerivadas | |
10.1101/2020.04.15.20067199 | |
The global shortage of reagents and kits for nucleic acid extraction and molecular detection of SARS-CoV-2, requires new cost-effective strategies for the diagnosis of suspected COVID-19 cases, especially in countries that need to increase detection capacity. Pooled nucleic acid testing has been extensively used as a cost-effective strategy for HIV, HepB, HepC and influenza. Also, protocols dispensing of RNA extraction appears as an attractive option for detection of SARS-CoV-2. In this study, pooling nasopharyngeal samples with both automated and manual extraction proved reliable, and thus a potential efficient alternative for the diagnosis of suspected COVID-19 in developing countries. | |
www.medrxiv.org | |
2020 | |
Artículo | |
https://www.medrxiv.org/content/medrxiv/early/2020/04/17/2020.04.15.20067199.full.pdf | |
Inglés | |
VIRUS RESPIRATORIOS | |
Aparece en las colecciones: | Artículos científicos |
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