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Optimizing RT-PCR detection of SARS-CoV-2 for developing countries using pool testing
Farfan Mauricio.
Torres Juan.
ORyan Miguel.
Olivares Mauricio.
Gallardo Pablo.
Salas Carolina.
Acceso Abierto
Atribución-NoComercial-SinDerivadas
10.1101/2020.04.15.20067199
The global shortage of reagents and kits for nucleic acid extraction and molecular detection of SARS-CoV-2, requires new cost-effective strategies for the diagnosis of suspected COVID-19 cases, especially in countries that need to increase detection capacity. Pooled nucleic acid testing has been extensively used as a cost-effective strategy for HIV, HepB, HepC and influenza. Also, protocols dispensing of RNA extraction appears as an attractive option for detection of SARS-CoV-2. In this study, pooling nasopharyngeal samples with both automated and manual extraction proved reliable, and thus a potential efficient alternative for the diagnosis of suspected COVID-19 in developing countries.
www.medrxiv.org
2020
Artículo
https://www.medrxiv.org/content/medrxiv/early/2020/04/17/2020.04.15.20067199.full.pdf
Inglés
VIRUS RESPIRATORIOS
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