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Evaluating the use of posterior oropharyngeal saliva in a point-of-care assay for the detection of SARS-CoV-2
Jonathan Hon Kwan Chen
Cyril Chik-Yan Yip
Rosana Wing-Shan Poon
Kwok Hung Chan
Ivan Fan Ngai Hung
Jasper Fuk-Woo Chan
Kwok_yung Yuen
Acceso Abierto
Nasopharyngeal swab
Point-of-care testing
During the Coronavirus disease 2019 (COVID-19) pandemic, logistic problems associated with specimen collection limited the SARS-CoV -2 testing especially in the community. In this study, we assessed the use of posterior oropharyngeal saliva as specimens for the detection of SARS-CoV -2 in an automated point-of-care molecular assay. Archived nasopharyngeal swab (NPS) and posterior oropharyngeal saliva specimens of 58 COVID-19 patients were tested with the Xpert® Xpress SARS-CoV -2 assay . SARS -CoV -2 was detected in either NPS or saliva specimens of all patients. Among them, 84.5% (49/58) tested positive in both NPS and saliva, 10.3% (6/58) tested positive in NPS only, and 5.2% (3/58) tested positive in saliva only. No significant difference in detection rate was observed between NPS and saliva (McNemar’s test p=0.5078). The detection rate was slightly higher for N2 (NPS 94.8% and Saliva 93.1%) than that of the E gene target (Saliva:89.7% vs 82.8%) on both specimen types. Significant earlier median Ct value was observed for NPS comparing to that of saliva on both E (26.8 vs 29.7, p=0.0002) and N2 gene target (29.3 vs 32.3, p=0.0002). The median Ct value of E gene target was significantly earlier than that of the N2 gene target for both NPS (26.8 vs 29.3, p<0.0001) and saliva (29.7 vs 32.3, p<0.0001). In conclusion, posterior oropharyngeal saliva and NPS were found to have similar detection rates in the point-of-care test for SARS-CoV-2 detection. Due to the easy collection of posterior oropharyngeal saliva, the use of saliva as an alternative specimen type for SARS-CoV-2 detection is recommended
Emerging Microbes & Infections
Epidemia COVID-19
Appears in Collections:Artículos científicos

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