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http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/1723| Development of a Novel, Genome Subtraction-Derived, SARS-CoV-2-Specific COVID-19-nsp2 Real-Time RT-PCR Assay and Its Evaluation Using Clinical Specimens | |
| Cyril Chik-Yan Yip Chi-Chun Ho Jasper Fuk-Woo Chan Kelvin To Shuk Ying Chan Cheuk Yiu WONG Raymond Tam Tom Wai-Hin Chung Kwok Hung Chan Ivan Fan Ngai Hung Owen Tak-Yin Tsang Stephen Kwok-Wing Tsui Kwok_yung Yuen | |
| Acceso Abierto | |
| Atribución | |
| https://doi.org/10.3390/ijms21072574 | |
| SARS-CoV-2 Real-time RT-PCR Genome subtraction Clinical evaluation Sensitivity COVID-19-nsp2 assay | |
| The pandemic novel coronavirus infection, Coronavirus Disease 2019 (COVID-19), has affected at least 190 countries or territories, with 465,915 confirmed cases and 21,031 deaths. In a containment-based strategy, rapid, sensitive and specific testing is important in epidemiological control and clinical management. Using 96 SARS-CoV-2 and 104 non-SARS-CoV-2 coronavirus genomes and our in-house program, GolayMetaMiner, four specific regions longer than 50 nucleotides in the SARS-CoV-2 genome were identified. Primers were designed to target the longest and previously untargeted nsp2 region and optimized as a probe-free real-time reverse transcription-polymerase chain reaction (RT-PCR) assay. The new COVID-19-nsp2 assay had a limit of detection (LOD) of 1.8 TCID50/mL and did not amplify other human-pathogenic coronaviruses and respiratory viruses. Assay reproducibility in terms of cycle threshold (Cp) values was satisfactory, with the total imprecision (% CV) values well below 5%. Evaluation of the new assay using 59 clinical specimens from 14 confirmed cases showed 100% concordance with our previously developed COVID-19-RdRp/Hel reference assay. A rapid, sensitive, SARS-CoV-2-specific real-time RT-PCR assay, COVID-19-nsp2, was developed. | |
| International Journal of Molecular Sciences | |
| 08-04-2020 | |
| Artículo | |
| https://www.mdpi.com/1422-0067/21/7/2574 | |
| Inglés | |
| Epidemia COVID-19 | |
| Investigadores | |
| VIRUS RESPIRATORIOS | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos científicos |
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