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http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/1631| S gene and 5a accessory gene are responsible for the attenuation of virulent infectious bronchitis coronavirus | |
| Zhao, Y Cheng, J Yan, S Jia, W Zhang, K Zhang, G | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| To explore the critical genes associated with infectious bronchitis virus (IBV) virulence, we compared the genome sequences of virulent YN strain and its attenuated strain aYN. Accumulation of mutations in the S gene and the accessory gene 5a were observed, suggesting a potential role in the loss of viral pathogenicity. Two recombinant IBVs (rIBVs) with replacement of the S gene or 5a with corresponding regions from aYN were rescued to verify this speculation. Embryo mortality time/rate showed that rYN-S-aYN and rYN-5a-aYN had an attenuated phenotype in ovo. Replication assay in ovo and in vitro demonstrated the rIBVs had similar replication patterns with wild-type rIBV. Both rIBVs showed reduced mortality, tissue lesions and tissue virus titers in chicken. In conclusion, S gene and 5a accessory gene are responsible for the attenuation of virulent IBV. Insight into the genes responsible for virus attenuation will facilitate the development of future vaccines against IBV. | |
| Virology | |
| 2019 | |
| Preimpreso | |
| https://coronavirus.1science.com/item/22dfcdb8a4eef20ddabdfba9f53f884c45fc8091 | |
| Inglés | |
| VIRUS RESPIRATORIOS | |
| Aparece en las colecciones: | Artículos científicos |
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| 110891.pdf | 2.31 MB | Adobe PDF | Visualizar/Abrir |