Please use this identifier to cite or link to this item: https://covid-19.conacyt.mx/jspui/handle/1000/1626
The human coronavirus HCoV-229E S-protein structure and receptor binding
Li, Z
Tomlinson, A
Wong, A
Zhou, D
Desforges, M
Talbot, P
Benlekbir, S
Rubinstein, J
Rini, J
Acceso Abierto
Atribución-NoComercial-SinDerivadas
The coronavirus S-protein mediates receptor binding and fusion of the viral and host cell membranes. In HCoV-229E, its receptor binding domain (RBD) shows extensive sequence variation but how S-protein function is maintained is not understood. Reported are the X-ray crystal structures of Class III-V RBDs in complex with human aminopeptidase N (hAPN), as well as the electron cryomicroscopy structure of the 229E S-protein. The structures show that common core interactions define the specificity for hAPN and that the peripheral RBD sequence variation is accommodated by loop plasticity. The results provide insight into immune evasion and the cross-species transmission of 229E and related coronaviruses. We also find that the 229E S-protein can expose a portion of its helical core to solvent. This is undoubtedly facilitated by hydrophilic subunit interfaces that we show are conserved among coronaviruses. These interfaces likely play a role in the S-protein conformational changes associated with membrane fusion.
ELife
2019
Preimpreso
https://coronavirus.1science.com/item/2b4c1aeefa3089f9e65578f5ebb0ecb21db2e5a3
Inglés
VIRUS RESPIRATORIOS
Appears in Collections:Artículos científicos

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