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http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/1619| A complete protocol for whole-genome sequencing of virus from clinical samples: Application to coronavirus OC43 | |
| Maurier, F Beury, D Fléchon, L Varré, J Touzet, H Goffard, A Hot, D Caboche, S | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| Genome sequencing of virus has become a useful tool for better understanding of virus pathogenicity and epidemiological surveillance. Obtaining virus genome sequence directly from clinical samples is still a challenging task due to the low load of virus genetic material compared to the host DNA, and to the difficulty to get an accurate genome assembly. Here we introduce a complete sequencing and analyzing protocol called V-ASAP for Virus Amplicon Sequencing Assembly Pipeline. Our protocol is able to generate the viral dominant genome sequence starting from clinical samples. It is based on a multiplex PCR amplicon sequencing coupled with a reference-free analytical pipeline. This protocol was applied to 11 clinical samples infected with coronavirus OC43 (HcoV-OC43), and led to seven complete and two nearly complete genome assemblies. The protocol introduced here is shown to be robust, to produce a reliable sequence, and could be applied to other virus. | |
| Virology | |
| 2019 | |
| Preimpreso | |
| https://coronavirus.1science.com/item/299ddfccc731c300190182307c5c35629301fb8a | |
| Inglés | |
| VIRUS RESPIRATORIOS | |
| Aparece en las colecciones: | Artículos científicos |
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| Fichero | Tamaño | Formato | |
|---|---|---|---|
| 110751.pdf | 1.43 MB | Adobe PDF | Visualizar/Abrir |