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http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/1493| Divergent coronaviruses detected in wild birds in Brazil, including a central park in São Paulo | |
| Barbosa, C Durigon, E Thomazelli, L Ometto, T Marcatti, R Nardi, M de Aguiar, D Pinho, J Petry, M Neto, I Serafini, P Rodrigues, R de Azevedo Junior, S Góes, L de Araujo, J | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| Coronaviruses are single-stranded positive-sense RNA viruses associated with important avian diseases. Their relatively high rates of mutation and recombination frequencies allow them to adapt to new hosts and ecological niches. Although Brazil has 18% of global avian species diversity, studies regarding the presence of avian viral diseases in wild birds in South America are scarce. In this study, we performed a retrospective analysis of the presence of CoVs in 746 wild birds. Oropharyngeal and cloacal swabs were obtained and placed together in vials containing VTM transport medium collected in different regions of Brazil between 2006 and 2013. Screening for viral nucleic acid was performed using conventional RT-PCR and pancoronavirus nested PCR. Positive samples were characterized by partial sequencing of the RNA-dependent RNA polymerase (RdRp) gene, and ensuing phylogenetic analysis was performed to investigate the association between virus epidemiology and bird migration routes. Coronavirus RNA were detected and sequenced from six samples, in which three were related to gammacoronaviruses group and the other three to deltacoronavirus group. Our study documents the presence of CoVs related to avian gamma- and deltacoronaviruses circulating in both urban- and poultry-farm regions of Brazil, implicating wild birds as potential carriers of CoVs which may represent a risk to poultry farms and public health in Brazil. | |
| Brazilian Journal of Microbiology | |
| 2019 | |
| Preimpreso | |
| https://coronavirus.1science.com/item/67c92fba247085c0efb73252e2660c2fd4ddd6a4 | |
| Inglés | |
| VIRUS RESPIRATORIOS | |
| Aparece en las colecciones: | Artículos científicos |
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