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http://conacyt.repositorioinstitucional.mx/jspui/handle/1000/1413| Sensitive and specific detection of low-level antibody responses in mild Middle East respiratory syndrome coronavirus infections | |
| Okba, N Stalin Raj, V Widjaja, I GeurtsvanKessel, C De Bruin, E Chandler, F Park, W Kim, N Farag, E Al-Hajri, M Bosch, B Oh, M Koopmans, M Reusken, C Haagmans, B | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| Middle East respiratory syndrome coronavirus (MERS-CoV) infections in humans can cause asymptomatic to fatal lower respiratory lung disease. Despite posing a probable risk for virus transmission, asymptomatic to mild infections can go unnoticed; a lack of seroconversion among some PCR-confirmed cases has been reported. We found that a MERS-CoV spike S1 protein-based ELISA, routinely used in surveillance studies, showed low sensitivity in detecting infections among PCR-confirmed patients with mild clinical symptoms and cross-reactivity of human coronavirus OC43-positive serum samples. Using in-house S1 ELISA and protein microarray, we demonstrate that most PCR-confirmed MERS-CoV case-patients with mild infections seroconverted; nonetheless, some of these samples did not have detectable levels of virus-neutralizing antibodies. The use of a sensitive and specific serologic S1-based assay can be instrumental in the accurate estimation of MERS-CoV prevalence. | |
| Emerging Infectious Diseases | |
| 2019 | |
| Preimpreso | |
| https://coronavirus.1science.com/item/2a5c478eca8e058333afcc9f33b069dfa256ba45 | |
| Inglés | |
| VIRUS RESPIRATORIOS | |
| Aparece en las colecciones: | Artículos científicos |
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| 108681.pdf | 2 MB | Adobe PDF | Visualizar/Abrir |