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Sensitive and specific detection of low-level antibody responses in mild Middle East respiratory syndrome coronavirus infections
Okba, N
Stalin Raj, V
Widjaja, I
GeurtsvanKessel, C
De Bruin, E
Chandler, F
Park, W
Kim, N
Farag, E
Al-Hajri, M
Bosch, B
Oh, M
Koopmans, M
Reusken, C
Haagmans, B
Acceso Abierto
Middle East respiratory syndrome coronavirus (MERS-CoV) infections in humans can cause asymptomatic to fatal lower respiratory lung disease. Despite posing a probable risk for virus transmission, asymptomatic to mild infections can go unnoticed; a lack of seroconversion among some PCR-confirmed cases has been reported. We found that a MERS-CoV spike S1 protein-based ELISA, routinely used in surveillance studies, showed low sensitivity in detecting infections among PCR-confirmed patients with mild clinical symptoms and cross-reactivity of human coronavirus OC43-positive serum samples. Using in-house S1 ELISA and protein microarray, we demonstrate that most PCR-confirmed MERS-CoV case-patients with mild infections seroconverted; nonetheless, some of these samples did not have detectable levels of virus-neutralizing antibodies. The use of a sensitive and specific serologic S1-based assay can be instrumental in the accurate estimation of MERS-CoV prevalence.
Emerging Infectious Diseases
Appears in Collections:Artículos científicos

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