Please use this identifier to cite or link to this item: https://covid-19.conacyt.mx/jspui/handle/1000/1367
A Fusion Peptide in the Spike Protein of MERS Coronavirus
Alsaadi, E
Neuman, B
Jones, I
Acceso Abierto
Atribución-NoComercial-SinDerivadas
Coronaviruses represent current and emerging threats for many species, including humans. Middle East respiratory syndrome-related coronavirus (MERS-CoV) is responsible for sporadic infections in mostly Middle Eastern countries, with occasional transfer elsewhere. A key step in the MERS-CoV replication cycle is the fusion of the virus and host cell membranes mediated by the virus spike protein, S. The location of the fusion peptide within the MERS S protein has not been precisely mapped. We used isolated peptides and giant unilamellar vesicles (GUV) to demonstrate membrane binding for a peptide located near the N-terminus of the S2 domain. Key residues required for activity were mapped by amino acid replacement and their relevance in vitro tested by their introduction into recombinant MERS S protein expressed in mammalian cells. Mutations preventing membrane binding in vitro also abolished S-mediated syncytium formation consistent with the identified peptide acting as the fusion peptide for the S protein of MERS-CoV.
Viruses
2019
Preimpreso
https://coronavirus.1science.com/item/8bff9aab0fc438824a83ad6eafbf61f0f809f731
Inglés
VIRUS RESPIRATORIOS
Appears in Collections:Artículos científicos

Upload archives


File SizeFormat 
108221.pdf5.77 MBAdobe PDFView/Open